scaffold 其实是基因组组装时的概念。涉及到的相关概念如下： reads：就是我们测序产生的短读序列，通常一代和三代的reads读长在几千到几万bp之间，二代的相对较短，平均是几十到几百bp。 contig：中文叫做重叠群，就是不同reads之间的overlap交叠区，拼接成的序列就是contig scaffold: 是比contig还要长的 ...
tracrRNA（在CRISPR-Cas9编辑技术中被优化并命名为gRNA scaffold），它负责与Cas9结合，与重复序列具有同源性。 crRNA为引导序列，约20个碱基，具有特异性。 其中，crRNA和tracrRNA通过局部碱基配对组合并与Cas9结合后，引导Cas9识别切割目标DNA序列 (图1)。
关于Cas12a crRNA的直接重复序列（DR区或Scaffold区）的具体序列，这个信息可以因物种来源的Cas12a酶而异。 通常，DR序列是crRNA中高度保守的部分，它在生物体的CRISPR阵列中重复出现，分隔每个spacer序列。
scaffold直接翻译过来是脚手架。 这个概念和“测末端”有关。 简单地说，你测序的时候筛选到了20kb长的片段，但是一代和二代都测不通，只能测两端很短的序列。 这时候你有了这个20kb的片段两端的序列，以及这两段序列之间的距离（20k）。
The compiler uses this specification to generate code that the application can use to create, read, update and delete database entries, effectively treating the template as a "scaffold" on which to build a more powerful application. 翻译过来就是： “脚手架”是一种元编程的方法，用于构建基于数据库的应用。
SgRNA scaffold设计:sgRNA scaffold是一个特定的RNA结构，它与SGRNA结合并促进Cas9蛋白的切劃活性通常，sgRNA scaffold的序列是已知的，并且可以在许多载体中找到。 2.准备Cas9载体 获取包含Cas9蛋白表达框的载体，这个载体可以是商业化的，也可以是实验室内部构建的。
Discover how easy it is to access the information you need with Which Scaffold Hazard Is Present In This Video. Our platform has been carefully designed to make digital reading more convenient, organized, and efficient...
The purpose of providing Which Scaffold Hazard Is Present In This Video in digital format is to remove barriers between readers and information. Whether you are pursuing academic studies, advancing your professional career, or simply exploring new subjects, our platform allows you to locate and access relevant materials with just a few simple clicks...
Accessing Which Scaffold Hazard Is Present In This Video is a straightforward process. Our website features a user-friendly interface, making it easy to search, preview, and download the resources that best match your needs. We believe that every reader deserves quick and reliable access to quality information without being overwhelmed by unnecessary complexity...
When you open Which Scaffold Hazard Is Present In This Video, you gain more than just a file you gain a trusted source of information. Each document within our collection is selected and organized to ensure authenticity and relevance. We are committed to providing materials that can be used confidently for research, study, and general knowledge development...
We understand that information seekers come from different backgrounds. Some users may be university students looking for study materials, while others may be professionals in need of technical documentation or manuals. With Which Scaffold Hazard Is Present In This Video and thousands of similar titles, we strive to meet the diverse needs of our global audience...
Our digital library offers a wide variety of content types from textbooks and scholarly papers to practical guides, tutorials, and reference works. This diversity allows users to explore countless subjects across multiple disciplines...
One of the key benefits of using our platform is flexibility. You can read Which Scaffold Hazard Is Present In This Video online directly from your browser or download it for offline reading...
In todays fast-paced digital era, convenience and accessibility are essential. That is why we have built our system to prioritize quick access to trustworthy content...
Beyond the technical aspects, we also believe in the importance of promoting lifelong learning. The availability of resources like Which Scaffold Hazard Is Present In This Video encourages readers to explore new topics, deepen their understanding, and expand their intellectual horizons...
Another defining feature of our service is its commitment to reliability. We know that accuracy matters when it comes to information...
The reading experience itself has been optimized for ease and comfort. Each eBook, such as Which Scaffold Hazard Is Present In This Video, features clear formatting, structured chapters, and navigable sections...
In addition to reading convenience, our platform provides a secure and safe environment...
Our digital library continues to grow and evolve. We regularly add new publications, revised editions, and updated materials to keep our users informed and inspired...
For educators, researchers, and students, Which Scaffold Hazard Is Present In This Video can serve as an essential tool for study and instruction...
Our system also supports multi-language content and cross-referenced materials, making it easier for users from different linguistic backgrounds to find what they need...
The ability to instantly access reliable documents like Which Scaffold Hazard Is Present In This Video highlights the benefits of digital transformation in education and information sharing...
We also recognize that learning is not limited to formal education. Many individuals seek to expand their knowledge independently...
We have built our website to be responsive, fast, and compatible with modern web technologies...
The presentation of each document is also carefully managed to ensure readability...
In addition, our collection is continuously expanding across numerous subjects...
As we continue to grow, our mission remains the same to make high-quality knowledge accessible, organized, and easy to use...
Our library is not only a source of books but also a community of readers who share a passion for learning...
Start your exploration today. Whether you want to study deeply, gain practical information, or simply satisfy your curiosity, Which Scaffold Hazard Is Present In This Video offers the perfect starting point...
Knowledge is powerful and with Which Scaffold Hazard Is Present In This Video, that power is within your reach...
Explore Which Scaffold Hazard Is Present In This Video today and enjoy the benefits of modern digital reading...